首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠生长激素(GH)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、细胞裂解物样本中的GH1含量。GH1 即生长激素 1 基因，它编码生长激素，对人体生长发育至关重要。相关研究聚焦其在调节生长、代谢等方面的机制，探究基因表达调控、信号通路作用等，希望借此深入理解疾病发生发展，为生长发育异常等疾病治疗提供新思路。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于小鼠模型中的内分泌机制研究、代谢相关疾病机制探索、生长调控实验以及体外药物或因子干预后GH分泌水平的评估，为生长发育、能量代谢等领域的科研实验提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gh1 ELISA Kit; Gh ELISA Kit; Somatotropin ELISA Kit; Growth hormone ELISA Kit
缩写：GH1
Uniprot No.：P06880
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, cell lysates
检测范围：3.12 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 87 | ||||
| Range % | 82-93 | |||||
| 1:200 | Average % | 99 | ||||
| Range % | 96-102 | |||||
| 1:400 | Average % | 87 | ||||
| Range % | 84-91 | |||||
| 1:800 | Average % | 84 | ||||
| Range % | 80-88 | |||||
回收率：
| The recovery of mouse GH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 90-96 | ||||
| EDTA plasma (n=4) | 107 | 103-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2002.4622.4982.4802.3011002.0842.0742.0791.900501.7171.7561.7371.558251.2621.2981.2801.10112.50.7960.7720.7840.6056.250.4040.4250.4150.2363.120.2870.2910.2890.11000.1780.1790.179 | ||||||
货期：3-5 working days
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