首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人β半乳糖苷酶(βGAL)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的GLB1含量。GLB1基因编码β-半乳糖苷酶，与多种疾病相关。研究发现，其突变会导致β-半乳糖苷酶功能缺陷，引发GM1神经节苷脂贮积症和黏多糖贮积症IVB型等疾病。对其研究机制主要聚焦在解析突变影响及探索酶替代治疗等方面。试剂盒检测范围为7.8 μlU/mL-500 μlU/mL，适用于基础科研中探索βGAL在细胞代谢调控、衰老标记物分析或疾病模型中的表达水平变化；通过尿液分析探究代谢异常机制，或利用组织匀浆研究特定病理条件下溶酶体功能状态；为生物医学领域研究βGAL相关分子机制及生物标志物筛选提供有效工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Acid beta galactosidase ELISA Kit; Acid beta-galactosidase ELISA Kit; Beta galactosidase 1 ELISA Kit; Beta galactosidase ELISA Kit; Beta-galactosidase ELISA Kit; BGAL_HUMAN ELISA Kit; EBP ELISA Kit; EBP; included ELISA Kit; Elastin receptor 1 (67kD) ELISA Kit; Elastin receptor 1 67kDa ELISA Kit; Elastin receptor 1 ELISA Kit; Elastin receptor 1; included ELISA Kit; Elastin-binding protein; included ELISA Kit; ELNR1 ELISA Kit; Galactosidase beta 1 ELISA Kit; GLB 1 ELISA Kit; GLB1 ELISA Kit; Lactase ELISA Kit; MPS4B ELISA Kit; S-GAL; included ELISA Kit
缩写：GLB1
Uniprot No.：P16278
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：7.8 μlU/mL-500 μlU/mL
灵敏度：1.95 μlU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human βGAL in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 100 | ||||
| Range % | 95-110 | |||||
| 1:10 | Average % | 97 | ||||
| Range % | 92-101 | |||||
| 1:20 | Average % | 86 | ||||
| Range % | 82-90 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of human βGAL spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-98 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| µlU/mlOD1OD2AverageCorrected 5002.0461.9521.9991.800 2501.7421.6651.7041.505 1251.3651.4041.3851.186 62.51.0571.1091.0830.884 31.20.8240.7860.8050.606 15.60.5110.5380.5250.326 7.80.3420.3290.3360.137 00.2010.1970.199 | ||||||
货期：3-5 working days
×
