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产品描述：人葡萄糖6磷酸异构酶(GPI)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPI含量。GPI是糖基磷脂酰肌醇的英文缩写。它是一种广泛存在于真核细胞膜上的锚定蛋白，参与细胞信号传导、细胞粘附等多种生理过程。研究显示，其在肿瘤发生发展、寄生虫感染等病理机制中发挥重要作用，是潜在的治疗靶点。试剂盒检测范围为12.5 ng/mL-800 ng/mL，本试剂盒通过高特异性抗体对靶标进行捕获与检测，可广泛应用于科研领域，如代谢通路研究、疾病相关生物标志物筛选、药物作用机制探索等场景，为体外实验提供精准的蛋白定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AMF ELISA Kit; Aurocrine motility factor ELISA Kit; Autocrine motility factor ELISA Kit; DKFZp686C13233 ELISA Kit; EC 5.3.1.9 ELISA Kit; G6PI_HUMAN ELISA Kit; Glucose phosphate isomerase ELISA Kit; Glucose-6-phosphate isomerase ELISA Kit; GNPI ELISA Kit; GPI ELISA Kit; Gpi1 ELISA Kit; Hexose monophosphate isomerase ELISA Kit; Hexosephosphate isomerase ELISA Kit; Neuroleukin ELISA Kit; NLK ELISA Kit; Oxoisomerase ELISA Kit; PGI ELISA Kit; PHI ELISA Kit; Phosphoglucose isomerase ELISA Kit; Phosphohexomutase ELISA Kit; Phosphohexose isomerase ELISA Kit; Phosphosaccharomutase ELISA Kit; SA 36 ELISA Kit; SA-36 ELISA Kit; SA36 ELISA Kit; Sperm antigen 36 ELISA Kit
缩写：GPI
Uniprot No.：P06744
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：12.5 ng/mL-800 ng/mL
灵敏度：3.12 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPI in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of human GPI spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 84-95 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected8002.0652.0762.0711.9684001.5801.5571.5691.4662000.8350.8240.8300.7271000.4320.4450.4390.336500.2850.2780.2820.179250.1900.1980.1940.09112.50.1390.1330.1360.03300.1020.1040.103 | ||||||
货期：3-5 working days
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