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产品描述：人谷胱甘肽过氧化物酶1(GPX1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPX1含量。GPX1（谷胱甘肽过氧化物酶1）是一种重要的抗氧化酶，主要存在于肺、肝、肾等氧张力较高的组织中。它在过氧化氢的解毒中起作用，是人体最重要的抗氧化酶之一。GPX1在肝癌组织中高表达，与肝癌的发生、发展及预后密切相关，可能作为肝癌筛选、预后预测的标志物。研究显示，GPX1共表达基因主要参与基因转录、翻译、蛋白泛素化等生物学过程，并参与TGF-β、mTOR、WNT等信号通路。试剂盒检测范围为15.6 μU/mL-1000 μU/mL，该产品可用于细胞氧化还原状态评估、抗氧化药物作用机制研究，以及心血管疾病、神经退行性疾病等与GPX1活性相关的科研领域，为体外实验提供高灵敏度的定量分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AL033363 ELISA Kit; Cellular glutathione peroxidase ELISA Kit; Glutathione peroxidase 1 ELISA Kit; Glutathione peroxidase ELISA Kit; GPx 1 ELISA Kit; GPx-1 ELISA Kit; GPX1 ELISA Kit; GPX1_HUMAN ELISA Kit; GPXD ELISA Kit; GSHPx-1 ELISA Kit; GSHPX1 ELISA Kit; MGC14399 ELISA Kit; MGC88245 ELISA Kit
缩写：GPX1
Uniprot No.：P07203
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 μU/mL-1000 μU/mL
灵敏度：3.9 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GPX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:200 | Average % | 91 | ||||
| Range % | 88-94 | |||||
| 1:400 | Average % | 105 | ||||
| Range % | 100-107 | |||||
| 1:800 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:1600 | Average % | 98 | ||||
| Range % | 94-103 | |||||
回收率：
| The recovery of human GPX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-98 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2AverageCorrected10002.6682.6452.6572.5445002.3552.3332.3442.2312501.9141.8871.9011.7881251.1231.1451.1341.02162.50.6560.6670.6620.54931.250.3780.3900.3840.27115.60.2610.2430.2520.13900.1150.1110.113 | ||||||
货期：3-5 working days
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