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产品描述：鱼谷胱甘肽过氧化物酶(GSH-PX)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GSH-PX含量。GSH-PX（谷胱甘肽过氧化物酶）是一种重要的抗氧化酶，能够清除体内的活性氧，保护细胞免受氧化损伤。其研究机制主要涉及GSH-PX在肝脏疾病中的作用，如慢性肝炎、非酒精性脂肪性肝病、酒精性肝病、肝硬化和肝癌等，通过调节氧化应激和脂质过氧化反应，影响疾病的发生和发展。试剂盒检测范围为375 μU/mL-6000 μU/mL，适用于鱼类生理学研究、环境污染物对机体氧化损伤评估、水产饲料抗氧化添加剂效果分析等科研领域，为水产养殖健康管理、生态毒理学研究及营养调控提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：GSH-PX
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：375 μU/mL-6000 μU/mL
灵敏度：187.5 μU/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:400 | Average % | 98 | ||||
| Range % | 93-103 | |||||
| 1:800 | Average % | 92 | ||||
| Range % | 87-97 | |||||
回收率：
| The recovery of fish GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 99 | 94-104 | ||||
| EDTA plasma (n=4) | 89 | 84-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μU/mlOD1OD2Average 60000.0890.0840.087 30000.2460.2650.256 15000.5510.5800.566 7500.9330.9810.957 3751.6461.5691.608 02.3622.3982.380 | ||||||
货期：3-5 working days
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