首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠谷胱甘肽过氧化酶(GSH-Px)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GSH-Px含量。GSH-Px（谷胱甘肽过氧化物酶）是一种广泛存在于生物体内的抗氧化酶，具有清除活性氧和过氧化氢的能力。其活性中心为硒半胱氨酸，通过催化GSH变为GSSG，将有毒的过氧化物还原成无毒的羟基化合物，保护细胞膜不受损害。GSH-Px的研究机制主要涉及其在抗氧化防御中的作用，以及与硒水平的关系。试剂盒检测范围为31.25 U/mL-2000 U/mL，适用于科研领域探究氧化应激机制，如代谢性疾病、神经退行性疾病或衰老相关研究中的GSH - Px动态变化分析，为评估抗氧化能力及氧化损伤修复提供有效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Gpx1 ELISA Kit; Glutathione peroxidase 1 ELISA Kit; GPx-1 ELISA Kit; GSHPx-1 ELISA Kit; EC 1.11.1.9 ELISA Kit; Cellular glutathione peroxidase ELISA Kit; Selenium-dependent glutathione peroxidase 1 ELISA Kit
缩写：GSH-Px
Uniprot No.：P11352
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 U/mL-2000 U/mL
灵敏度：7.8 U/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse GSH-PX in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 91 | |||||
| Range % | 85-97 | ||||||
| 1:2 | Average % | 94 | |||||
| Range % | 89-98 | ||||||
| 1:4 | Average % | 97 | |||||
| Range % | 91-102 | ||||||
| 1:8 | Average % | 95 | |||||
| Range % | 89-99 | ||||||
回收率：
| The recovery of mouse GSH-PX spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 97 | 93-102 | |||||
| EDTA plasma (n=4) | 88 | 82-94 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| U/mlOD1OD2AverageCorrected20002.3242.4012.3632.27510001.6021.6991.6511.5635001.0561.0391.0480.9602500.6570.6650.6610.5731250.4310.4460.4390.35162.50.2740.2850.2800.19231.250.1580.1620.1600.07200.0870.0880.088 | |||||||
货期：3-5 working days
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