首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肾损伤分子1(Kim-1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、尿液样本中的HAVCR1含量。HAVCR1，全称甲型肝炎病毒细胞受体1，是一种跨膜糖蛋白，在急性肾损伤和哮喘、过敏等疾病中发挥重要作用。HAVCR1作为HAV的受体，在病毒感染过程中起关键作用，其研究机制涉及病毒与受体的相互作用、信号传导等过程。试剂盒检测范围为0.9 ng/mL-60 ng/mL，广泛应用于小鼠急性肾损伤模型构建、药物肾毒性评估、肾脏疾病机制研究等科研领域，尤其适用于动态监测肾损伤修复过程中Kim-1的表达水平变化，为探索肾脏疾病的分子调控网络提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Havcr1 ELISA Kit; Kim1 ELISA Kit; Tim1 ELISA Kit; Timd1 ELISA Kit; Hepatitis A virus cellular receptor 1 homolog ELISA Kit; HAVcr-1 ELISA Kit; Kidney injury molecule 1 ELISA Kit; KIM-1 ELISA Kit; T cell immunoglobulin and mucin domain-containing protein 1 ELISA Kit; TIMD-1 ELISA Kit; T cell membrane protein 1 ELISA Kit; T-cell immunoglobulin mucin receptor 1 ELISA Kit; TIM-1 ELISA Kit; CD antigen CD365 ELISA Kit
缩写：HAVCR1
Uniprot No.：Q5QNS5
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates, urine
检测范围：0.9 ng/mL-60 ng/mL
灵敏度：0.225 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Microbiology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 88-98 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 90-101 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 92-99 | |||||
回收率：
| The recovery of mouse Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-97 | ||||
| EDTA plasma (n=4) | 94 | 88-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected602.3562.3452.3512.183301.7861.7251.7561.588151.2641.2361.2501.0827.50.8130.8010.8070.6393.750.5130.4890.5010.3331.80.3780.3630.3710.2030.90.2670.2510.2590.09100.1700.1660.168 | ||||||
货期：3-5 working days
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