首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人组蛋白去乙酰化酶9(HDAC9)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HDAC9含量。HDAC9即组蛋白去乙酰化酶9，在基因表达调控、细胞增殖、分化等生理过程中发挥作用。研究发现其异常表达与肿瘤、心血管疾病等多种疾病的发生发展相关，机制主要涉及调控相关基因的表达、影响细胞信号通路等。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于基础医学研究中HDAC9在疾病发生发展中的功能探索，例如心血管病理机制分析、肿瘤微环境研究或神经系统疾病模型中蛋白表达水平的动态监测。试剂盒支持多种样本类型检测，为体外实验、动物模型组织分析及细胞分子机制研究提供灵活选择本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HD 7 ELISA Kit; HD 7B ELISA Kit; HD 9 ELISA Kit; HD7 ELISA Kit; HD7B ELISA Kit; HD9 ELISA Kit; HDAC 7 ELISA Kit; HDAC 7B ELISA Kit; HDAC 9 ELISA Kit; HDAC 9B ELISA Kit; HDAC 9FL ELISA Kit; HDAC ELISA Kit; HDAC7 ELISA Kit; HDAC7B ELISA Kit; HDAC9 ELISA Kit; HDAC9_HUMAN ELISA Kit; HDAC9B ELISA Kit; HDAC9FL ELISA Kit; HDRP ELISA Kit; Histone deacetylase 4/5 related protein ELISA Kit; Histone deacetylase 7 ELISA Kit; Histone deacetylase 7B ELISA Kit; Histone deacetylase 9 ELISA Kit; Histone deacetylase 9A ELISA Kit; Histone deacetylase related protein ELISA Kit; Histone deacetylase-related protein ELISA Kit; KIAA0744 ELISA Kit; MEF2 interacting transcription repressor MITR ELISA Kit; MEF2 interacting transcription repressor protein ELISA Kit; MEF2-interacting transcription repressor MITR ELISA Kit; MITR ELISA Kit
缩写：HDAC9
Uniprot No.：Q9UKV0
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HDAC9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 85-94 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 95-109 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 86-92 | |||||
回收率：
| The recovery of human HDAC9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 86-94 | ||||
| EDTA plasma (n=4) | 99 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.3112.4222.3672.22651.9021.8991.9011.7602.51.3231.4251.3741.2331.250.9651.0240.9950.8540.6250.6580.6330.6460.5050.3120.3440.3720.3580.2170.1560.2570.2660.2620.12100.1350.1460.141 | ||||||
货期：3-5 working days
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