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产品描述：人HEXIM1蛋白(HEXIM1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HEXIM1含量。HEXIM1蛋白是一种RNA结合蛋白，在RNA转录加工中起关键作用。研究发现，HEXIM1在多种癌症中表达异常，与肿瘤的发生发展密切相关。其具体机制包括调节P-TEFb活性、影响病毒转录以及与R环调控相关等。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于体外实验研究，例如探索HEXIM1在肿瘤发生、干细胞分化或HIV病毒潜伏机制中的表达变化，也可用于药物筛选模型中评估化合物对HEXIM1信号通路的调控作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Cardiac lineage protein 1 ELISA Kit; CLP 1 ELISA Kit; CLP1 ELISA Kit; EDG 1 ELISA Kit; EDG1 ELISA Kit; Estrogen down-regulated gene 1 protein ELISA Kit; FLJ13562 ELISA Kit; Hexamethylene bis acetamide inducible 1 ELISA Kit; Hexamethylene bis acetamide inducible protein ELISA Kit; Hexamethylene bis acetamide inducible transcript 1 ELISA Kit; Hexamethylene bis-acetamide-inducible protein 1 ELISA Kit; HEXI1_HUMAN ELISA Kit; HEXIM 1 ELISA Kit; Hexim1 ELISA Kit; HEXIM1 protein ELISA Kit; HIS 1 ELISA Kit; HIS1 ELISA Kit; HMBA inducible ELISA Kit; MAQ 1 ELISA Kit; MAQ1 ELISA Kit; Menage a quatre 1 ELISA Kit; Menage a quatre protein 1 ELISA Kit; Protein HEXIM1 ELISA Kit
缩写：HEXIM1
Uniprot No.：O94992
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HEXIM1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 81-95 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 90-105 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 91-110 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 80-100 | |||||
回收率：
| The recovery of human HEXIM1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 82-98 | ||||
| EDTA plasma (n=4) | 97 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 20002.1392.0522.0961.873 10001.9571.8161.8871.664 5001.5431.5041.5241.301 2501.0631.0391.0510.828 1250.7250.7040.7150.492 62.50.5420.5190.5310.308 31.250.3230.3090.3160.093 00.2290.2170.223 | ||||||
货期：3-5 working days
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