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产品描述：鱼肝细胞生长因子(HGF)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的HGF含量。HGF（肝细胞生长因子）是一种多功能细胞因子，主要来源于肝脏的Kupffer细胞、内皮细胞等，能刺激肝细胞增殖，并作用于上皮细胞、造血细胞、血管内皮细胞等多种细胞。HGF通过旁分泌或自分泌机制，在胚胎发生、创伤愈合、血管发生、组织器官再生等方面发挥重要作用。试剂盒检测范围为200 pg/mL-8000 pg/mL，适用于基础医学研究领域，例如肝脏疾病模型中的HGF动态监测、药物干预效果评价或肿瘤微环境相关研究，也可用于细胞培养上清液中HGF分泌水平的定量分析，为探索HGF在病理生理过程中的调控机制提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HGF
Uniprot No.：F1REK1
种属：Fish
样本类型：serum, plasma
检测范围：230 pg/mL-8000 pg/mL
灵敏度：145 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<15% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish HGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 95 | |||||
| Range % | 89-105 | ||||||
| 1:2 | Average % | 89 | |||||
| Range % | 85-100 | ||||||
| 1:4 | Average % | 89 | |||||
| Range % | 82-100 | ||||||
| 1:8 | Average % | 93 | |||||
| Range % | 87-99 | ||||||
回收率：
| The recovery of fish HGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 93 | 88-98 | |||||
| EDTA plasma (n=4) | 95 | 89-102 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2Average 80000.1760.1820.179 40000.3810.3790.380 20000.5580.5650.562 6850.8860.9020.894 2301.5021.4881.495 02.4152.4362.426 | |||||||
货期：3-5 working days
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