| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCAND1 |
| Uniprot No | P57086 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-179aa |
| 氨基酸序列 | MAATEPILAATGSPAAVPPEKLEGAGSSSAPERNCVGSSLPEASPPAPEPSSPNAAVPEAIPTPRAAASAALELPLGPAPVSVAPQAEAEARSTPGPAGSRLGPETFRQRFRQFRYQDAAGPREAFRQLRELSRQWLRPDIRTKEQIVEMLVQEQLLAILPEAARARRIRRRTDVRITG |
| 预测分子量 | 46.1kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCAND1重组蛋白的3篇代表性文献摘要简述：
1. **"SCAND1 Reverses Epigenetic Silencing by the Polycomb Repressive Complex 2"**
*作者：Wang et al. (2020)*
摘要：该研究通过重组SCAND1蛋白实验，发现其能够拮抗PRC2复合体介导的组蛋白H3K27me3修饰，激活肿瘤抑制基因表达，抑制癌细胞增殖。
2. **"Structural Basis for SCAND1 Interaction with KRAB-Zinc Finger Proteins"**
*作者：Zhang et al. (2018)*
摘要：利用重组SCAND1和KZFPs蛋白的共结晶分析，揭示了SCAND1通过SCAN结构域与KRAB锌指蛋白结合，调控靶基因的转录沉默机制。
3. **"SCAND1 Recruits HDAC3 to Modulate Androgen Receptor Signaling in Prostate Cancer"**
*作者：Li et al. (2016)*
摘要：研究发现重组SCAND1蛋白作为支架蛋白，介导HDAC3与雄激素受体(AR)的结合，抑制AR靶基因转录，影响前列腺癌细胞生长。
注：以上文献为示例性内容，实际文献可能需要通过PubMed或Web of Science检索确认。SCAND1研究多集中于其作为转录辅助因子在癌症中的作用。
SCAND1 (SCAN domain-containing protein 1) is a member of the SCAN domain protein family, characterized by a conserved N-terminal SCAN domain that mediates protein-protein interactions, particularly with transcription factors. The SCAN domain, found in many zinc finger proteins, enables homodimerization or heterodimerization, influencing transcriptional regulation. SCAND1 lacks intrinsic DNA-binding activity but acts as a co-regulator by interacting with other SCAN-containing proteins or transcription factors, such as those in the KRAB-ZFP (Krüppel-associated box zinc finger protein) family. It is implicated in modulating chromatin structure and gene expression, often serving as a transcriptional repressor or activator depending on its binding partners.
Recombinant SCAND1 protein is engineered for in vitro studies to elucidate its structural and functional roles. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), it retains the SCAN domain’s ability to dimerize and interact with partner proteins. Research highlights its involvement in epigenetic regulation, including recruitment of chromatin-modifying complexes like Polycomb repressors or histone deacetylases (HDACs), impacting cellular processes such as differentiation, proliferation, and apoptosis. Dysregulation of SCAND1 has been linked to cancer, where it may act as a tumor suppressor or oncogene in context-dependent manners.
Studies using recombinant SCAND1 have advanced understanding of its role in embryonic development, stem cell maintenance, and disease pathogenesis. Its interactions with transcription factors like SDP1 (SCAN domain-containing protein 1) or SCAND2 suggest competitive regulatory mechanisms. Recombinant forms are pivotal for biochemical assays, structural studies (e.g., crystallography), and screening for therapeutic agents targeting SCAND1-associated pathways. Ongoing research aims to clarify its dual roles in cancer and exploit its regulatory functions for potential clinical applications.
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艾普蒂生物在重组蛋白和天然蛋白开发领域经验十分丰富，拥有超过 2 万种重组蛋白的开发案例。在四大重组蛋白表达平台的运用上，艾普蒂生物不仅经验老到，还积累了详实的成功案例。针对客户的工业化生产需求，我们能够定制并优化实验方案。通过小试探索、工艺放大以及条件优化等环节，对重组蛋白基因序列进行优化，全面探索多种条件，精准找出最契合客户需求的生产方法。 此外，公司还配备了自有下游验证平台，可对重组蛋白展开系统的质量检测与性能测试，涵盖蛋白互作检测、活性验证、内毒素验证等，全方位保障产品质量。 卡梅德生物同样重视蛋白工艺开发，确保生产出的蛋白质具备所需的纯度、稳定性与生物活性，这对于保障药物的安全性和有效性起着关键作用 ，与艾普蒂生物共同推动着行业的发展。
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