| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAR1A |
| Uniprot No | Q9NR31 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-198aa |
| 氨基酸序列 | MSFIFEWIYNGFSSVLQFLGLYKKSGKLVFLGLDNAGKTTLLHMLKDDRLGQHVPTLHPTSEELTIAGMTFTTFDLGGHEQARRVWKNYLPAINGIVFLVDCADHSRLVESKVELNALMTDETISNVPILILGNKIDRTDAISEEKLREIFGLYGQTTGKGNVTLKELNARPMEVFMCSVLKRQGYGEGFRWLSQYID |
| 预测分子量 | 49.4kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SAR1A重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**: "Structural basis of Sar1-mediated membrane budding by the COPII coat"
**作者**: Stagg, S.M. et al.
**摘要**: 本研究解析了SAR1A重组蛋白与COPII复合体的结构，揭示了SAR1A在GTP结合状态下如何诱导内质网膜出芽的分子机制，阐明了其在囊泡运输中的构象变化及调控作用。
2. **文献名称**: "Recombinant expression and functional characterization of SAR1A GTPase in endoplasmic reticulum export"
**作者**: Bi, X. & Mancias, J.D.
**摘要**: 通过大肠杆菌表达系统获得重组SAR1A蛋白，验证其GTP酶活性及与Sec23/Sec24复合体的相互作用，证实SAR1A在ER至高尔基体运输中的关键调控功能。
3. **文献名称**: "SAR1A mutations impair vesicular transport and contribute to developmental disorders"
**作者**: Vaz, F.M. et al.
**摘要**: 利用重组SAR1A蛋白进行体外功能实验，发现特定突变导致其GTP水解能力缺陷，进而破坏COPII囊泡形成，与神经发育异常及脂代谢疾病相关。
以上文献均聚焦SAR1A重组蛋白的结构、功能及病理机制，涵盖基础研究与疾病关联方向。
**Background of SAR1A Recombinant Protein**
SAR1A is a member of the SAR1 family of small GTPases, which play a pivotal role in intracellular protein trafficking. Specifically, SAR1A is a key regulator of the COPII (coat protein complex II)-mediated vesicle transport system, responsible for shuttling newly synthesized proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. As a GTPase, SAR1A cycles between an active GTP-bound state and an inactive GDP-bound state, a process tightly controlled by guanine nucleotide exchange factors (GEFs) and GTPase-activating proteins (GAPs). Upon activation, SAR1A recruits COPII components to the ER membrane, initiating vesicle formation and cargo selection—a critical step in maintaining cellular secretory pathways and homeostasis.
Recombinant SAR1A protein is produced through genetic engineering techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. Its recombinant form retains the structural and functional properties of the native protein, enabling researchers to study its role in vesicle biogenesis, membrane dynamics, and protein sorting *in vitro*. SAR1A dysfunction has been linked to various diseases, including metabolic disorders, neurodegenerative conditions, and cancers, where disrupted ER-Golgi trafficking contributes to pathogenesis.
Studies using recombinant SAR1A have advanced structural biology efforts, such as elucidating its GTP-binding domain and interaction sites with COPII components. Additionally, it serves as a tool for drug discovery, particularly in targeting secretory pathway abnormalities. Overall, SAR1A recombinant protein is indispensable for dissecting fundamental mechanisms of cellular transport and developing therapeutic strategies for trafficking-related disorders.
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