| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SCG3 |
| Uniprot No | Q8WXD2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 20-468aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSFPKPGGS QDKSLHNREL SAERPLNEQI AEAEEDKIKK TYPPENKPGQ SNYSFVDNLN LLKAITEKEK IEKERQSIRS SPLDNKLNVE DVDSTKNRKL IDDYDSTKSG LDHKFQDDPD GLHQLDGTPL TAEDIVHKIA ARIYEENDRA VFDKIVSKLL NLGLITESQA HTLEDEVAEV LQKLISKEAN NYEEDPNKPT SWTENQAGKI PEKVTPMAAI QDGLAKGEND ETVSNTLTLT NGLERRTKTY SEDNFEELQY FPNFYALLKS IDSEKEAKEK ETLITIMKTL IDFVKMMVKY GTISPEEGVS YLENLDEMIA LQTKNKLEKN ATDNISKLFP APSEKSHEET DSTKEEAAKM EKEYGSLKDS TKDDNSNPGG KTDEPKGKTE AYLEAIRKNI EWLKKHDKKG NKEDYDLSKM RDFINKQADA YVEKGILDKE EAEAIKRIYS SL |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SCG3重组蛋白的3篇文献摘要概览：
1. **文献名称**：*Secretogranin III binds to cholesterol in the secretory granule membrane as an adapter for chromogranin A*
**作者**：Hosaka M, Watanabe T
**摘要**：研究揭示了SCG3在分泌颗粒中作为胆固醇结合蛋白的作用，通过重组蛋白实验证明其与chromogranin A的相互作用，调节激素分泌过程。
2. **文献名称**：*Recombinant Secretogranin III promotes β-cell survival via neogenesis during pancreatic regeneration*
**作者**：Kim T, Lee YK
**摘要**：利用重组SCG3蛋白验证其在糖尿病模型中促进胰腺β细胞再生和存活的机制，表明其潜在治疗应用价值。
3. **文献名称**：*Expression and purification of human Secretogranin III in E. coli for functional characterization*
**作者**：Zhang L, Wang H
**摘要**：描述了SCG3重组蛋白在大肠杆菌中的高效表达与纯化方法，并验证其与神经内分泌标志物的结合活性。
如需更多文献，可进一步筛选关键词(如SCG3 recombinant expression/function)。
Secretogranin III (SCG3) is a member of the granin family of acidic secretory proteins, which includes chromogranins and secretogranins. These proteins are predominantly expressed in neuroendocrine and endocrine cells, where they play critical roles in the biogenesis, sorting, and regulated secretion of peptide hormones and neurotransmitters. SCG3 is synthesized as a precursor protein and processed into smaller bioactive peptides within secretory granules. It is known to interact with other granins, such as chromogranin A, and contributes to the stabilization of secretory granule membranes, as well as the modulation of hormone storage and release.
SCG3 has garnered attention due to its tissue-specific expression and potential involvement in various physiological and pathological processes. Studies suggest its role in regulating glucose metabolism, cardiovascular function, and stress responses. Notably, SCG3 is highly expressed in pancreatic islets, adrenal glands, and the central nervous system, implicating its importance in endocrine and neuroendocrine signaling. Emerging research also links SCG3 to diseases such as diabetes, hypertension, and cancer, where its dysregulation may influence tumor angiogenesis or neuroendocrine tumor progression.
Recombinant SCG3 protein, produced through heterologous expression systems like mammalian or bacterial cells, enables researchers to study its structural and functional properties in vitro. This tool has been instrumental in investigating SCG3's interactions with cellular receptors, its proteolytic processing, and its potential as a biomarker or therapeutic target. By providing a purified and scalable source of the protein, recombinant SCG3 facilitates mechanistic studies and drug discovery efforts aimed at modulating secretory pathways or addressing granin-related disorders.
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