Recombinant Human SCF protein

中文名： 干细胞因子配体(SCF)重组蛋白
别名： KITLG;MGF;SCF;Kit ligand

货号: PA1000-2843

Price： ￥询价

20μg 50μg 100μg ＞100μg

数量：

大包装询价

产品详情

纯度	>95%SDS-PAGE.
种属	Human
靶点	SCF
Uniprot No	P21583
内毒素	< 0.01EU/μg
表达宿主	E.coli
表达区间	26-190aa
氨基酸序列	EGICRNRVTN NVKDVTKLVA NLPKDYMITL KYVPGMDVLP SHCWISEMVV QLSDSLTDLL DKFSNISEGL SNYSIIDKLV NIVDDLVECV KENSSKDLKK SFKSPEPRLF TPEEFFRIFN RSIDAFKDFV VASETSDCVV SSTLSPEKDS RVSVTKPFML PPVAA
预测分子量	19 kDa
蛋白标签	His tag N-Terminus
缓冲液	PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
稳定性 & 储存条件	Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months.
复溶	Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

参考文献

以下是3篇与SCF(Stem Cell Factor)重组蛋白相关的代表性文献概览：

1. **标题**：*"Crystal structure of the SCF complex (c-Kit-SCF)"*

**作者**：Martin, F.H. et al.

**摘要**：报道了SCF与其受体c-Kit复合物的晶体结构，揭示了SCF二聚化介导受体激活的分子机制，为重组SCF的功能研究提供结构基础。

2. **标题**：*"Expression and functional analysis of recombinant human stem cell factor in Escherichia coli"*

**作者**：Langjahr, P. et al.

**摘要**：描述通过大肠杆菌表达系统生产重组人SCF，验证其生物活性可促进造血祖细胞增殖，为低成本大规模生产提供方案。

3. **标题**：*"SCF enhances ex vivo expansion of hematopoietic stem cells using cytokine combinations"*

**作者**：Broudy, V.C.

**摘要**：研究重组SCF与其他细胞因子(如TPO、FLT3L)联用对造血干细胞的体外扩增效果，证明其在再生医学中的应用潜力。

注：SCF重组蛋白研究多集中于1990-2010年，近年更多关注其工程化改造(如聚乙二醇化SCF)。建议通过PubMed或Web of Science以"recombinant SCF protein"、"stem cell factor expression"为关键词获取最新进展。

背景信息

Stem cell factor (SCF), also known as KIT ligand or steel factor, is a critical cytokine that plays a central role in regulating cellular survival, proliferation, and differentiation. It functions by binding to the KIT receptor tyrosine kinase, activating downstream signaling pathways such as PI3K/AKT and MAPK. Naturally produced by stromal cells, SCF exists in both membrane-bound and soluble forms, influencing processes like hematopoiesis, melanogenesis, and germ cell development. Its interaction with KIT is essential for the maintenance of hematopoietic stem cells, mast cells, and melanocytes.

Recombinant SCF proteins are engineered using genetic modification techniques, typically expressed in prokaryotic (e.g., *E. coli*) or eukaryotic systems (e.g., mammalian cells). Bacterial expression yields non-glycosylated proteins, while mammalian systems produce glycosylated forms closer to native human SCF. These recombinant variants retain bioactivity while enabling scalable production for research and therapeutic applications. Commercial SCF products often undergo rigorous purification to eliminate endotoxins and host proteins, ensuring consistency in experimental or clinical settings.

In biomedical research, recombinant SCF is widely used to expand hematopoietic progenitor cells *in vitro* and enhance engraftment efficiency in bone marrow transplantation. Clinically, it has been combined with granulocyte colony-stimulating factor (G-CSF) to mobilize stem cells into peripheral blood for collection. Emerging applications include tissue engineering, where SCF supports stem cell maintenance in synthetic niches, and targeted cancer therapies exploring KIT signaling inhibition. Challenges persist in optimizing delivery methods, minimizing off-target effects, and understanding context-dependent signaling outcomes. Despite these hurdles, recombinant SCF remains a cornerstone tool in both basic science and translational medicine.