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产品描述：牛结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的HP含量。牛结合珠蛋白（Haptoglobin，Hp）是一种在哺乳动物血清中广泛存在的酸性糖蛋白，主要功能是与游离血红蛋白结合，引导其进入降解代谢程序，防止其对机体的损伤。其亚型与多种疾病如感染、糖尿病、肿瘤等存在相关性，具有作为治疗药物的潜力。牛结合珠蛋白的分离纯化方法包括硫酸铵沉淀、聚乙二醇沉淀等，这些方法有助于大规模纯化结合珠蛋白，为后续的药物开发提供材料。试剂盒检测范围为7.81 ng/mL-2000 ng/mL，该产品适用于畜牧科研中炎症相关疾病的机制研究、疫苗或药物干预效果评估，以及动物健康监测等场景，为牛群疾病模型建立、营养代谢研究提供关键数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HPHaptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
缩写：HP
Uniprot No.：Q2TBU0
种属：Bos taurus (Bovine)
样本类型：serum, plasma
检测范围：7.81 ng/mL-2000 ng/mL
灵敏度：7.81 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cardiovascular
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1000 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2000 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:4000 | Average % | 103 | ||||
| Range % | 99-112 | |||||
| 1:8000 | Average % | 98 | ||||
| Range % | 94-102 | |||||
回收率：
| The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 92 | 88-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 20000.2430.2520.248 5000.4120.4360.424 1200.7930.7540.774 31.251.2141.2391.227 7.811.7951.7241.760 02.4052.3592.382 | ||||||
货期：3-5 working days
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