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产品描述:山羊结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒为竞争法ELISA试剂盒,定量检测血清、血浆样本中的Hpt/HP含量。试剂盒检测范围为3.9 ng/mL-1000 ng/mL,该产品可广泛应用于动物医学研究、免疫学实验或畜牧科学领域,支持科研人员探究山羊在应激状态、疾病模型或药物干预下Hpt/HP的动态变化,为相关生理病理机制研究提供可靠工具。本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
缩写:Hpt/HP
种属:Capra hircus (Goat)
样本类型:serum, plasma
检测范围:3.9 ng/mL-1000 ng/mL
灵敏度:1.95 ng/mL
反应时间:1-5h
样本体积:50-100ul
检测波长:450 nm
研究领域:Cardiovascular
测定原理:quantitative
测定方法:Competitive
精密度:
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of goat haptoglobin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:100 | Average % | 91 | ||||
Range % | 84-99 | |||||
1:200 | Average % | 95 | ||||
Range % | 90-101 | |||||
1:400 | Average % | 97 | ||||
Range % | 87-101 | |||||
1:800 | Average % | 94 | ||||
Range % | 88-98 |
回收率:
The recovery of goat haptoglobin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-101 | ||||
EDTA plasma (n=4) | 96 | 91-102 | ||||
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
ng/mlOD1OD2Average 10000.3110.3130.312 2500.5730.5860.580 62.50.9350.9480.942 15.61.5811.5961.589 3.91.9862.0051.996 02.1822.2362.209 |
货期:3-5 working days
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