首页 / 产品 / ELISA试剂盒 / 其他ELISA试剂盒
产品描述：牛热休克蛋白HSP90α(HSP90AA1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HSP90AA1含量。HSP90AA1 是重要的热休克蛋白，在细胞应激反应、蛋白稳态维持等方面发挥关键作用。它与肿瘤发生发展密切相关，能稳定多种致癌蛋白。研究聚焦于其抑制机制，通过阻断其功能影响下游信号通路，为肿瘤治疗提供新策略。试剂盒检测范围为6.25 ng/mL-400 ng/mL，适用于科研领域中对牛源样本中HSP90α的定量检测，例如在热应激模型、炎症反应或代谢疾病研究中评估蛋白表达动态变化，亦可用于细胞培养条件下应激相关分子机制的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HSP90AA1 ELISA Kit; HSP90A ELISA Kit; HSPCA ELISA Kit; Heat shock protein HSP 90-alpha ELISA Kit
缩写：HSP90AA1
Uniprot No.：Q76LV2
种属：Bos taurus (Bovine)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：6.25 ng/mL-400 ng/mL
灵敏度：1.56 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine HSP90AA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-95 | |||||
| 1:2 | Average % | 102 | ||||
| Range % | 97-107 | |||||
| 1:4 | Average % | 91 | ||||
| Range % | 85-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-103 | |||||
回收率：
| The recovery of bovine HSP90AA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 95 | 88-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected4002.1922.1782.1852.0292001.6841.6561.6701.5141001.1071.1211.1140.958500.6510.6370.6440.488250.4620.4820.4720.31612.50.3280.3310.3300.1746.250.2410.2510.2460.09000.1550.1570.156 | ||||||
货期：3-5 working days
×
