Chicken Heat Shock Protein 90,HSP-90 ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

货号: IPD-E12873C

Price： ￥2500

48T 96T

数量：

大包装询价

产品描述：鸡热休克蛋白90(HSP-90)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的HSP90AA1含量。HSP90AA1，也称为热休克蛋白90α，是一种分子伴侣，参与细胞周期控制和信号转导。其研究机制涉及通过ATP酶活性诱导靶蛋白构象变化，激活靶蛋白。HSP90AA1在多种生理和病理过程中发挥重要作用，如细胞应激、炎症和癌症。试剂盒检测范围为1.56 ng/mL-100 ng/mL，该产品广泛用于家禽热应激机制研究、疾病模型建立、环境应激评估等领域，特别适合实验室开展家禽生理调控、抗逆性育种及药效评价等科研项目，为揭示HSP - 90在生物体应激响应中的分子机制提供可靠工具，所有应用仅限于科学研究用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：HSP90AA1 ELISA Kit; HSPCAHeat shock protein HSP 90-alpha ELISA Kit
缩写：HSP90AA1
Uniprot No.：P11501
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken HSP-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-103
1:2	Average %	89
	Range %	85-96
1:4	Average %	93
	Range %	83-99
1:8	Average %	95
	Range %	89-98

回收率：

The recovery of chicken HSP-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	95	87-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/mlOD1OD2AverageCorrected1002.4282.3882.4082.303501.9941.9351.9651.860251.3621.3341.3481.24312.50.8300.8680.8490.7446.250.6180.5850.6020.4973.120.3450.3420.3440.2391.560.2510.2620.2570.15200.1030.1070.105