首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠热休克蛋白90(HSP-90)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSP90AA1含量。HSP90AA1 是一种重要的分子伴侣蛋白。它在细胞内参与众多信号通路和蛋白质的折叠、稳定。研究发现其与肿瘤发生发展密切相关，干扰其功能可使肿瘤相关蛋白降解，有望成为肿瘤治疗新靶点，目前对其深入研究有助于开发有效抗癌疗法。试剂盒检测范围为0.156 ng/mL-10 ng/mL，本试剂盒适用于体外定量检测小鼠来源样本，可支持基础科研中HSP - 90表达水平分析，如探究药物干预、环境胁迫或疾病模型下该蛋白的动态变化，为分子机制研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Hsp90aa1 ELISA Kit; Hsp86 ELISA Kit; Hsp86-1 ELISA Kit; HspcaHeat shock protein HSP 90-alpha ELISA Kit; Heat shock 86 kDa ELISA Kit; HSP 86 ELISA Kit; HSP86 ELISA Kit; Tumor-specific transplantation 86 kDa antigen ELISA Kit; TSTA ELISA Kit
缩写：HSP90AA1
Uniprot No.：P07901
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse Hsp-90 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 81-101 | |||||
| 1:10 | Average % | 95 | ||||
| Range % | 86-104 | |||||
| 1:20 | Average % | 95 | ||||
| Range % | 82-105 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 82-101 | |||||
回收率：
| The recovery of mouse Hsp-90 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-105 | ||||
| EDTA plasma (n=4) | 95 | 89-110 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7732.7872.7802.66551.8421.7401.7911.6762.51.1051.1641.1351.0201.250.6680.6730.6710.5560.6250.3980.4030.4010.2860.3120.2820.2660.2740.1590.1560.1590.1520.1560.04100.1120.1180.115 | ||||||
货期：3-5 working days
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