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产品描述：人热休克蛋白90α（HSP-90α）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSP90AA1含量。HSP90AA1是重要的靶点，属于热休克蛋白90家族成员。它在细胞内蛋白折叠、稳定和降解等过程起关键作用，参与细胞生长、增殖和存活等重要生理过程。研究其机制有助于理解肿瘤等疾病发生发展，也为开发针对性治疗药物提供方向。试剂盒检测范围为0.78 ng/mL-50 ng/mL，适用于体外实验研究，可广泛应用于肿瘤生物学、细胞应激反应机制、药物靶点筛选等领域的科研工作，为探索HSP - 90α在疾病发生发展中的作用提供可靠工具，同时支持生物标志物相关研究的样本定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Heat shock 86 kDa ELISA Kit; Heat shock protein 90kDa alpha cytosolic class A member 1 ELISA Kit; Heat shock protein 90kDa alpha cytosolic class B member 1 ELISA Kit; Heat shock protein HSP 90 alpha ELISA Kit; Heat shock protein HSP 90 beta ELISA Kit; Heat shock protein HSP 90-alpha ELISA Kit; HS90A_HUMAN ELISA Kit; HSP 84 ELISA Kit; HSP 86 ELISA Kit; Hsp 90 ELISA Kit; HSP86 ELISA Kit; HSP90A ELISA Kit; HSP90AA1 ELISA Kit; HSP90AB1 ELISA Kit; HSP90B ELISA Kit; HSPC1 ELISA Kit; HSPC2 ELISA Kit; HSPCAL1 ELISA Kit; HSPCAL4 ELISA Kit; Renal carcinoma antigen NY-REN-38 ELISA Kit
缩写：HSP-90α
Uniprot No.：P07900
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human HSP-90α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 93 | ||||
| Range % | 90-95 | |||||
| 1:10 | Average % | 104 | ||||
| Range % | 100-107 | |||||
| 1:20 | Average % | 94 | ||||
| Range % | 89-97 | |||||
| 1:40 | Average % | 99 | ||||
| Range % | 92-103 | |||||
回收率：
| The recovery of human HSP-90α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-97 | ||||
| EDTA plasma (n=4) | 94 | 91-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.7122.6992.7062.569252.0132.0022.0081.87112.51.4001.4221.4111.2746.250.8040.7880.7960.6593.120.4240.4280.4260.2891.560.3490.3410.3450.2080.780.2450.2340.2400.10300.1390.1350.137 | ||||||
货期：3-5 working days
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