首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠热休克蛋白70(HSP-70)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的HSPA1A含量。HSPA1A是重要的热休克蛋白成员，在细胞应激反应中起关键作用，其表达变化与多种疾病尤其癌症密切相关。研究机制主要围绕其对细胞内蛋白折叠、运输及细胞凋亡的调控等方面，通过调节其功能有望为相关疾病治疗提供新策略。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，可应用于热休克反应机制探索、药物干预效果评估、疾病模型（如心血管损伤、神经退行性疾病）中应激水平变化的动态监测等基础研究领域，其兼容血清、血浆及组织匀浆样本的特性为不同实验设计提供灵活选择。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：HSPA1A
Uniprot No.：P0DMW0
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Neuroscience
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat hSP-70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 92 | ||||
| Range % | 88-95 | |||||
| 1:200 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:400 | Average % | 92 | ||||
| Range % | 86-96 | |||||
| 1:800 | Average % | 98 | ||||
| Range % | 94-101 | |||||
回收率：
| The recovery of rat hSP-70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 91-97 | ||||
| EDTA plasma (n=4) | 95 | 91-98 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.4022.3332.3682.22620001.9461.9261.9361.79410001.4221.4331.4281.2865000.9110.9240.9180.7762500.4770.4910.4840.3421250.3320.3230.3280.18662.50.2440.2370.2410.09900.1430.1410.142 | ||||||
货期：3-5 working days
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