首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人免疫球蛋白结合蛋白（BIP）酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、组织匀浆样本中的HSPA5含量。HSPA5，全称热休克蛋白5，是一种内质网应激的关键因子，参与蛋白质折叠、组装和质量控制。其在炎症性肠病中通过调节内质网应激、介导细胞凋亡和激活炎症信号通路等机制影响肠上皮细胞的存活和凋亡。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究细胞或动物模型中内质网应激通路的动态分析、疾病发生发展机制探索，以及药物或基因干预下BIP表达变化的定量评估；为科研实验提供稳定、高特异性的检测方案，助力于蛋白质稳态调控及相关疾病机制的深入研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：78 kDa glucose regulated protein ELISA Kit; 78 kDa glucose-regulated protein ELISA Kit; AL022860 ELISA Kit; AU019543 ELISA Kit; BIP ELISA Kit; D2Wsu141e ELISA Kit; D2Wsu17e ELISA Kit; Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 ELISA Kit; Endoplasmic reticulum lumenal Ca2+ binding protein grp78 ELISA Kit; Epididymis secretory sperm binding protein Li 89n ELISA Kit; FLJ26106 ELISA Kit; Glucose Regulated Protein 78 ELISA Kit; Glucose Regulated Protein 78kDa ELISA Kit; GRP 78 ELISA Kit; GRP-78 ELISA Kit; GRP78 ELISA Kit; GRP78_HUMAN ELISA Kit; Heat shock 70 kDa protein 5 ELISA Kit; Heat Shock 70kDa Protein 5 ELISA Kit; Heat shock protein family A (Hsp70) member 5 ELISA Kit; HEL S 89n ELISA Kit; Hsce70 ELISA Kit; HSPA 5 ELISA Kit; HSPA5 ELISA Kit; Immunoglobulin Heavy Chain Binding Protein ELISA Kit; Immunoglobulin heavy chain-binding protein ELISA Kit; mBiP ELISA Kit; MIF2 ELISA Kit; Sez7 ELISA Kit
缩写：HSPA5
Uniprot No.：P11021
种属：Homo sapiens (Human)
样本类型：serum, tissue homogenates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human BIP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 87-100 | |||||
| 1:2 | Average % | 89 | ||||
| Range % | 84-95 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-90 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of human BIP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 83-96 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.7522.7892.7712.64852.1612.1982.1802.0572.51.6361.6161.6261.5031.251.0681.0341.0510.9280.6250.6520.6120.6320.5090.3120.4370.4470.4420.3190.1560.3410.3690.3550.23200.1210.1240.123 | ||||||
货期：3-5 working days
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