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产品描述：人DNA结合蛋白抑制剂ID-1(ID1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的ID1含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，该产品适用于肿瘤机制研究、干细胞分化模型分析、血管生成实验等科研领域，支持体外培养细胞、实验动物模型及人类生物样本中的 ID1 蛋白表达水平检测，为探索 ID1 在疾病发生发展中的分子机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：bHLHb24 ELISA Kit; Class B basic helix-loop-helix protein 24 ELISA Kit; dJ857M17.1.2 (inhibitor of DNA binding 1, dominant negative helix-loop-helix protein) ELISA Kit; DNA binding protein inhibitor ID 1 ELISA Kit; DNA binding protein inhibitor ID1 ELISA Kit; DNA-binding protein inhibitor ID-1 ELISA Kit; Dominant negative helix loop helix protein ELISA Kit; ID 1 ELISA Kit; ID ELISA Kit; ID1 ELISA Kit; ID1_HUMAN ELISA Kit; Inhibitor of Differentiation 1 ELISA Kit; Inhibitor of DNA binding 1 ELISA Kit; inhibitor of DNA binding 1, dominant negative helix-loop-helix protein ELISA Kit
缩写：ID1
Uniprot No.：P41134
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Epigenetics and Nuclear Signaling
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ID1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 82-93 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 94-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 90-104 | |||||
| 1:8 | Average % | 85 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of human ID1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-95 | ||||
| EDTA plasma (n=4) | 101 | 96-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6542.6472.6512.50110002.2222.3272.2752.1255001.5671.5441.5561.4062500.7850.7450.7650.6151250.4370.4330.4350.28562.50.3580.3470.3530.20331.250.2460.2540.2500.10000.1510.1480.150 | ||||||
货期：3-5 working days
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