首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人异锁链素(IDES)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的IDES含量。试剂盒检测范围为47 pg/mL-3000 pg/mL，科研人员可利用探索IDES在器官纤维化模型中的表达特征，分析其在炎症性疾病动物或细胞实验中的动态变化，或通过检测体液样本评估其作为生物标志物的潜在价值，为疾病机制研究提供可靠工具。适用于体外研究场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IDES
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：47 pg/mL-3000 pg/mL
灵敏度：11.75 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Others
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IDES in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 85 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 94-103 | |||||
| 1:8 | Average % | 86 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of human IDES spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 100 | 95-105 | ||||
| EDTA plasma (n=4) | 99 | 94-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected30002.2912.2872.2892.15615001.7521.7571.7551.6227501.0911.1141.1030.9703750.6750.6980.6870.554187.50.4380.4570.4480.31593.750.2970.2820.2900.157470.2250.2350.2300.09700.1330.1320.133 | ||||||
货期：3-5 working days
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