首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠吲哚胺2,3-双加氧酶1(IDO1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IDO1含量。IDO1，即吲哚胺2，3-双加氧酶-1，是一种关键限速酶，催化色氨酸降解，参与肿瘤免疫逃逸，与肿瘤恶性转化和患者预后差密切相关。其抑制剂研究进展迅速，有望成为肿瘤免疫治疗的新靶点。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，可广泛应用于基础科研领域，例如探究肿瘤免疫治疗中 IDO1 的动态变化、评估炎症性疾病模型中酶活性调控机制，或用于药物开发中抑制剂功效的体外评价。为研究 IDO1 在免疫代谢通路中的功能提供可靠工具，助力免疫调控机制与干预策略的探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Ido1 ELISA Kit; Ido ELISA Kit; Indo ELISA Kit; Indoleamine 2,3-dioxygenase 1 ELISA Kit; IDO-1 ELISA Kit; EC 1.13.11.52 ELISA Kit; Indoleamine-pyrrole 2,3-dioxygenase ELISA Kit
缩写：IDO1
Uniprot No.：P28776
种属：Mus musculus (Mouse)
样本类型：serum, plasma, tissue homogenates
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Metabolism
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse IDO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-94 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-101 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 87-95 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of mouse IDO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 93 | 89-98 | ||||
| EDTA plasma (n=4) | 99 | 95-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 40002.8783.1122.9952.869 20002.2172.3742.2962.170 10001.4751.5351.5051.379 5000.9330.9530.9430.817 2500.5710.6180.5950.469 1250.3720.4010.3870.261 62.50.2610.2600.2610.135 00.1210.1300.126 | ||||||
期：3-5 working days
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