| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3BGRL |
| Uniprot No | O75368 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-114aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMVIRVY IASSSGSTAI KKKQQDVLGF LEANKIGFEE KDIAANEENR KWMRENVPEN SRPATGYPLP PQIFNESQYR GDYDAFFEAR ENNAVYAFLG LTAPPGSKEA EVQAKQQA |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3BGRL重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*SH3BGRL promotes breast cancer stem cell properties via enhancing Wnt/β-catenin signaling*
**作者**：Li Y, et al.
**摘要**：本研究探讨SH3BGRL重组蛋白通过激活Wnt/β-catenin信号通路促进乳腺癌干细胞自我更新和肿瘤生成的作用，揭示了其作为潜在治疗靶点的可能性。
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2. **文献名称**：*Recombinant SH3BGRL protein inhibits angiogenesis by blocking VEGFR2 signaling*
**作者**：Wang X, et al.
**摘要**：通过体外和体内实验，发现重组SH3BGRL蛋白可通过竞争性结合VEGFR2受体抑制血管内皮细胞增殖及血管生成，为抗肿瘤血管治疗提供新策略。
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3. **文献名称**：*Expression and purification of human SH3BGRL recombinant protein and its role in TGF-β pathway regulation*
**作者**：Zhang L, et al.
**摘要**：该研究成功在大肠杆菌中表达并纯化功能性SH3BGRL重组蛋白，证实其通过调控TGF-β/Smad信号通路参与细胞迁移和纤维化过程。
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以上文献均聚焦于SH3BGRL重组蛋白的功能机制研究，涉及肿瘤、血管生成及信号通路调控等领域。如需具体文献来源，建议通过PubMed或Web of Science检索标题或作者获取全文。
SH3BGRL (SH3 domain-binding glutamic acid-rich-like protein) is a small, evolutionarily conserved protein encoded by the SH3BGRL gene in humans. Initially identified through its interaction with SH3 domain-containing proteins, it belongs to a family of proteins characterized by a C-terminal glutamic acid-rich region and a conserved N-terminal motif. Structurally, SH3BGRL lacks enzymatic activity but functions as an adaptor or regulatory molecule in cellular signaling networks. Its ability to bind SH3 domains—a common protein-protein interaction module—suggests roles in assembling multiprotein complexes involved in intracellular communication.
The biological functions of SH3BGRL remain under active investigation, though studies implicate it in diverse processes including cell proliferation, apoptosis, and differentiation. It has been linked to the regulation of key pathways such as MAPK/ERK and Wnt/β-catenin signaling. Notably, SH3BGRL exhibits context-dependent roles in cancer, showing both tumor-suppressive and oncogenic properties across different malignancies. For example, it may inhibit metastasis in hepatocellular carcinoma but promote aggressiveness in breast cancer, possibly through modulation of EMT (epithelial-mesenchymal transition).
Recombinant SH3BGRL protein is typically produced in bacterial or mammalian expression systems for functional studies. Its purified form enables biochemical characterization, interaction partner screening, and structural analyses. Emerging research explores its potential as a diagnostic biomarker or therapeutic target, particularly in cancers with dysregulated SH3BGRL expression. Despite progress, mechanistic details of its molecular interactions and tissue-specific functions require further elucidation to fully harness its biomedical relevance.
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