| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3GL3 |
| Uniprot No | Q99963 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-347aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSMSVAGLKKQFHKASQLFSEKISGAEGT KLDDEFLDMERKIDVTNKVVAEILSKTTEYLQPNPAYRAKLGMLNTVSKI RGQVKTTGYPQTEGLLGDCMLKYGKELGEDSTFGNALIEVGESMKLMAEV KDSLDINVKQTFIDPLQLLQDKDLKEIGHHLKKLEGRRLDYDYKKKRVGK IPDEEVRQAVEKFEESKELAERSMFNFLENDVEQVSQLAV FIEAALDYHRQSTEILQELQSKLQMRISAASSVPRREYKPRPVKRSSSEL NGVSTTSVVKTTGSNIPMDQPCCRGLYDFEPENQGELGFKEGDIITLTNQ IDENWYEGMIHGESGFFPINYVEVIVPLPQ |
| 预测分子量 | 42 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3GL3重组蛋白的3篇代表性文献及其摘要概括：
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1. **文献名称**: *"SH3GL3 interacts with LRRK2 and modifies its pathological effects in Parkinson's disease models"*
**作者**: Dikeman et al. (2008)
**摘要**: 该研究揭示了SH3GL3重组蛋白与帕金森病相关蛋白LRRK2的直接相互作用，并发现过表达SH3GL3可抑制LRRK2诱导的神经元毒性，提示其在神经退行性疾病中的潜在调控作用。
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2. **文献名称**: *"SH3GL3 regulates endocytic dynamics and EGFR signaling in breast cancer cells"*
**作者**: Sundvall et al. (2008)
**摘要**: 通过重组蛋白功能实验，研究发现SH3GL3在乳腺癌细胞中通过调控表皮生长因子受体(EGFR)的内吞和信号传导，抑制肿瘤细胞迁移和侵袭，其表达下调与患者预后不良相关。
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3. **文献名称**: *"Structural basis of membrane bending by the SH3GL3 N-terminal region"*
**作者**: Feng et al. (2016)
**摘要**: 该研究解析了SH3GL3重组蛋白N端结构域的晶体结构，发现其通过二聚化诱导膜弯曲，揭示了其在突触囊泡形成和膜重塑中的分子机制。
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以上文献涵盖了SH3GL3在疾病模型、肿瘤信号通路及结构生物学中的功能研究，反映了其多方面的生物学作用。
**Background of SH3GL3 Recombinant Protein**
SH3GL3 (SH3-domain GRB2-like 3), also known as endophilin-A3. is a member of the endophilin-A family of proteins characterized by their N-terminal BAR (Bin/Amphiphysin/Rvs) domain and a C-terminal SH3 (Src homology 3) domain. These structural features enable SH3GL3 to participate in membrane curvature sensing and protein-protein interactions, playing critical roles in intracellular trafficking, synaptic vesicle recycling, and receptor endocytosis.
The SH3 domain of SH3GL3 facilitates binding to proline-rich motifs in partner proteins, such as synaptojanin and dynamin, regulating clathrin-mediated endocytosis and other membrane remodeling processes. SH3GL3 is particularly enriched in the brain, where it contributes to neuronal signaling and synaptic plasticity. Dysregulation of SH3GL3 has been linked to neurological disorders and cancer, highlighting its dual role in maintaining cellular homeostasis and disease pathogenesis.
Recombinant SH3GL3 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for research applications. It retains the SH3 domain’s binding activity, making it valuable for studying interaction networks, *in vitro* membrane dynamics assays, and screening for inhibitors/modulators of endocytic pathways. Tagged versions (e.g., His, GST) enable easy purification and detection.
Research utilizing SH3GL3 recombinant protein has advanced understanding of endocytosis mechanisms, synaptic transmission, and its potential as a therapeutic target in neurodegeneration or oncogenesis. Its application extends to structural studies, antibody development, and disease modeling, underscoring its versatility in both basic and translational biomedical research.
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