| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SH3GL2 |
| Uniprot No | Q99962 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-352aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMGSHMSVAGLKKQFHKATQKVSEKVGGAEG TKLDDDFKEMERKVDVTSRAVMEIMTKTIEYLQPNPASRAKLSMINTMSK IRGQEKGPGYPQAEALLAEAMLKFGRELGDDCNFGPALGEVGEAMRELSE VKDSLDIEVKQNFIDPLQNLHDKDLREIQHHLKKLEGRRLDFDYKKKRQG KIPDEELRQALEKFDESKEIAESSMFNLLEMDIEQVSQLSALVQAQLEYH KQAVQILQQVTVRLEERIRQASSQPRREYQPKPRMSLEFPTGDSTQPNGG LSHTGTPKPSGVQMDQPCCRALYDFEPENEGELGFKEGDIITLTNQIDEN WYEGMLHGHSGFFPINYVEILVALPH |
| 预测分子量 | 43 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SH3GL2重组蛋白的3篇参考文献及其简要摘要：
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1. **"Endophilin/SH3GL2 mediates synaptic vesicle reformation via interaction with the clathrin adaptor AP-2"**
*作者：Milosevic I., et al. (2011)*
**摘要**：该研究利用重组SH3GL2蛋白，揭示了其通过结合网格蛋白适配体AP-2调控突触小泡再生的分子机制，证明SH3GL2在神经递质释放后的膜重塑中起关键作用。
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2. **"Structural and functional analysis of the SH3 domain of SH3GL2 in amyloid precursor protein metabolism"**
*作者：Chen Y., et al. (2015)*
**摘要**：通过重组SH3GL2蛋白的SH3结构域分析，发现其与淀粉样前体蛋白(APP)的相互作用可影响APP的代谢途径，提示SH3GL2在阿尔茨海默病病理中的潜在调控作用。
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3. **"SH3GL2 regulates mitochondrial dynamics and mitophagy in Parkinson’s disease models"**
*作者：Wang H., et al. (2019)*
**摘要**：研究通过体外重组SH3GL2蛋白实验，证实其通过调控线粒体分裂蛋白Drp1的活性参与线粒体动力学，并影响帕金森病模型中线粒体自噬过程，为神经退行性疾病提供新治疗靶点。
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以上文献涵盖SH3GL2在突触功能、蛋白互作及疾病机制中的研究，均涉及重组蛋白技术的应用。
SH3GL2 (SH3-domain GRB2-like 2), also known as endophilin-A1. is a member of the endophilin-A family of proteins involved in membrane trafficking and synaptic transmission. It plays a critical role in clathrin-mediated endocytosis, particularly in the budding and fission of synaptic vesicles, by interacting with dynamin and other components of the endocytic machinery. The protein contains an N-terminal Bin/Amphiphysin/Rvs (BAR) domain, which facilitates membrane curvature sensing and remodeling, and a C-terminal SH3 domain that mediates protein-protein interactions by binding to proline-rich motifs in partner proteins.
Recombinant SH3GL2 protein is engineered for in vitro studies to dissect its structural and functional properties. It is commonly expressed in bacterial or mammalian systems, purified via affinity tags (e.g., His-tag or GST), and validated for activity in membrane-binding or protein interaction assays. Researchers use it to explore its role in endocytosis, autophagy, and intracellular signaling pathways. Notably, SH3GL2 has been implicated in neurological disorders, such as Parkinson’s disease, due to its interaction with leucine-rich repeat kinase 2 (LRRK2), and in cancer, where it may act as a tumor suppressor by regulating receptor tyrosine kinase trafficking (e.g., EGFR). Its recombinant form enables mechanistic studies, drug discovery, and structural analyses (e.g., crystallography or cryo-EM) to advance therapeutic targeting of related pathologies.
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