首页 / 产品 / ELISA试剂盒 / 小鼠ELISA试剂盒
产品描述：小鼠肠脂肪酸结合蛋白(iFABP)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的iFABP含量。iFABP即肠脂肪酸结合蛋白，是一种小分子胞内蛋白。在背景上它与肠道脂肪酸摄取、转运等密切相关。研究机制方面，其可作为肠道损伤早期敏感标志物，还能参与脂肪酸代谢，影响细胞内信号转导及基因表达等。试剂盒检测范围为6.25 pg/mL-400 pg/mL，适用于肠道疾病动物模型研究，例如肠炎、缺血再灌注损伤等病理机制探索，也可用于体外细胞实验中评估药物或外界刺激对肠道上皮细胞的影响，为肠道屏障功能、代谢调控等基础研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Fabp2 ELISA Kit; FabpiFatty acid-binding protein ELISA Kit; intestinal ELISA Kit; Fatty acid-binding protein 2 ELISA Kit; Intestinal-type fatty acid-binding protein ELISA Kit; I-FABP ELISA Kit
缩写：iFABP
Uniprot No.：P55050
种属：Mus musculus (Mouse)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：6.25 pg/mL-400 pg/mL
灵敏度：1.56 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of mouse iFABP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 102 | ||||
| Range % | 96-108 | |||||
| 1:200 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:400 | Average % | 96 | ||||
| Range % | 91-102 | |||||
| 1:800 | Average % | 86 | ||||
| Range % | 82-92 | |||||
回收率：
| The recovery of mouse iFABP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 82-94 | ||||
| EDTA plasma (n=4) | 96 | 90-104 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 4002.2172.3172.2672.163 2001.3671.4671.4171.313 1000.7770.7970.7870.683 500.4120.4220.4170.313 250.2650.2680.2670.163 12.50.1890.1990.1940.090 6.250.1620.1650.1640.060 00.1040.1030.104 | ||||||
货期：3-5 working days
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