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产品描述：人干扰素α诱导蛋白-27，线粒体(IFI27)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFI27含量。IFI27是一种重要靶点。它属于干扰素诱导蛋白家族，与免疫应答密切相关。研究显示IFI27可能通过调节细胞信号通路、影响细胞增殖与凋亡等机制发挥作用，在肿瘤、免疫相关疾病等领域的研究逐渐增多，有望成为潜在治疗靶点。试剂盒检测范围为28 pg/mL-1800 pg/mL，本试剂盒可兼容多种生物样本类型，适用于体外检测人源性样本中IFI27的蛋白表达量，为科研领域研究病毒感染机制、免疫调节功能、疾病相关生物标志物筛选等提供定量分析工具。适用于实验室基础研究与药物开发中的靶标分子定量分析。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFI27; Interferon alpha-inducible protein 27, mitochondrial; p27; Interferon alpha-induced 11.5 kDa protein; Interferon-stimulated gene 12a protein; ISG12(a; ISG12A
缩写：IFI27
Uniprot No.：P40305
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：28 pg/mL-1800 pg/mL
灵敏度：7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IFI27 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 90-99 | |||||
| 1:2 | Average % | 86 | ||||
| Range % | 82-93 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 85-97 | |||||
回收率：
| The recovery of human IFI27 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 87 | 84-90 | ||||
| EDTA plasma (n=4) | 99 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected18002.6302.4582.5442.4119002.1262.2222.1742.0414501.3941.5111.4531.3202250.7680.7850.7770.6441120.5120.5570.5350.402560.3420.3210.3320.199280.2380.2450.2420.10900.1340.1310.133 | ||||||
货期：3-5 working days
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