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产品描述：猪α干扰素(IFN-α)酶联免疫试剂盒，为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IFNA1含量。IFNA1 为干扰素α1，是 I 型干扰素家族成员。其背景上在抗病毒、免疫调节等方面有重要作用。研究机制表明它能与细胞表面受体结合，激活下游信号通路，诱导多种抗病毒蛋白产生，从而抑制病毒复制、调节免疫细胞活性。试剂盒检测范围为7.8 pg/mL-500 pg/mL，适用于体外实验研究，如病毒感染模型中的细胞因子动态分析、免疫调节剂效果评估或疫苗免疫原性研究；动态监测对研究猪类病毒感染机制、免疫应答评估及疫苗研发具有重要意义。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IFN-α
Uniprot No.：Q6VAB8
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：7.8 pg/mL-500 pg/mL
灵敏度：1.95 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 89 | ||||
| Range % | 80-99 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-105 | |||||
| 1:8 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of pig IFN-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-102 | ||||
| EDTA plasma (n=4) | 97 | 90-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected5002.8952.8522.8742.7392502.1142.1172.1161.9811251.2541.2601.2571.12262.50.8460.8390.8430.70831.20.4890.4540.4720.33715.60.3010.3190.3100.1757.80.2570.2490.2530.11800.1330.1370.135 | ||||||
货期：3-5 working days
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