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产品描述：鸭γ干扰素(IFN-γ)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFN-含量。IFN-（干扰素相关靶点）在免疫调节中意义重大。其背景在于它与机体抗病毒、抗肿瘤等免疫反应密切相关。研究机制方面，主要是通过与细胞表面受体结合，激活相关信号通路，诱导多种抗病毒、免疫调节蛋白的表达，以发挥生物学效应。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于科研场景中鸭类病毒感染模型研究、疫苗免疫效果评价、免疫调节分子通路探索等方向本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
缩写：IFN-
Uniprot No.：Q9YGB9
种属：Duck
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of duck IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 91 | ||||
| Range % | 86-98 | |||||
| 1:2 | Average % | 98 | ||||
| Range % | 92-106 | |||||
| 1:4 | Average % | 85 | ||||
| Range % | 81-86 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of duck IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 103 | 101-107 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected 10002.2352.1992.2172.072 5001.5281.6551.5921.447 2501.1871.1201.1541.009 1250.7340.7860.7600.615 62.50.5550.5500.5530.408 31.20.3460.3240.3350.190 15.60.2040.2080.2060.061 00.1400.1490.145 | ||||||
货期：3-5 working days
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