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产品描述：山羊免疫球蛋白A(IgA)酶联免疫试剂盒，竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgA含量。IgA即免疫球蛋白A，是人体黏膜免疫的重要组成部分，在抵御病原体入侵等方面发挥关键作用。相关研究机制主要围绕其结构与功能的关系，探索如何增强IgA的免疫活性、研发基于IgA靶点的新型治疗方法，以应对感染性疾病、自身免疫病等。试剂盒检测范围为29 ng/mL-7500 ng/mL，该产品适用于动物免疫学研究，如疫苗接种效果评价、病原感染后免疫应答监测、营养干预对黏膜免疫的影响分析等方向，也可用于比较医学中反刍动物疾病模型的免疫机制探索。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgA
种属：Capra hircus (Goat)
样本类型：serum, plasma
检测范围：29 ng/mL-7500 ng/mL
灵敏度：29 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of goat immunoglobulin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5000 | Average % | 94 | ||||
| Range % | 90-103 | |||||
| 1:10000 | Average % | 96 | ||||
| Range % | 92-100 | |||||
| 1:20000 | Average % | 90 | ||||
| Range % | 85-97 | |||||
| 1:40000 | Average % | 92 | ||||
| Range % | 85-103 | |||||
回收率：
| The recovery of goat immunoglobulin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 90-100 | ||||
| EDTA plasma (n=4) | 95 | 91-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 75000.2890.3120.301 18750.5580.5420.550 4680.9120.9210.917 1171.6121.5431.578 291.8531.9341.894 02.4062.3982.402 | ||||||
货期：3-5 working days
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