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产品描述：牛免疫球蛋白A(IgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IgA含量。IgA 即免疫球蛋白 A，是人体免疫系统的重要组成部分，在黏膜免疫中发挥关键作用。研究围绕其结构与功能展开，探索其识别病原体机制，以及在免疫调节中的角色。IgA 相关研究为感染性疾病、自身免疫疾病诊断与治疗带来新方向。试剂盒检测范围为0.39 μg/mL-25 μg/mL，适用于研究牛群免疫状态、疫苗效力评估及肠道健康机制等科研领域；畜牧免疫学研究、疫苗开发验证、病原感染模型建立等场景；为牛免疫相关基础研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgA
种属：Bos taurus (Bovine)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.39 μg/mL-25 μg/mL
灵敏度：0.39 μg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of bovine IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 91 | ||||
| Range % | 86-99 | |||||
| 1:200 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:400 | Average % | 85 | ||||
| Range % | 81-87 | |||||
| 1:800 | Average % | 89 | ||||
| Range % | 83-94 | |||||
回收率：
| The recovery of bovine IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 92 | 87-95 | ||||
| EDTA plasma (n=4) | 97 | 92-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| μg/mlOD1OD2Average 250.1990.1870.193 12.50.3250.3290.327 6.250.4660.4890.478 3.120.7310.7600.746 1.561.0171.1051.061 0.781.3101.4101.360 0.391.5261.5701.548 02.1252.1622.144 | ||||||
货期：3-5 working days
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