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产品描述：豚鼠γ干扰素(IFN-γ)酶联免疫试剂盒双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFN-γ含量。IFN-γ即γ-干扰素，是具有多种生物学活性的细胞因子。背景上，它在免疫调节等机体生理功能中扮演重要角色。研究机制方面，其可激活巨噬细胞、增强NK细胞活性等，在抗感染、抗肿瘤及免疫相关疾病研究中受广泛关注。试剂盒检测范围为18.75 pg/mL-1200 pg/mL，尤其适用于豚鼠模型的感染性疾病机制探索和疫苗开发等科研领域；适用于评估免疫激活状态或炎症反应强度；该产品广泛应用于基础医学、兽医学研究中宿主免疫应答分析、病原体感染模型建立以及生物制剂效果评价等非诊断性科研场景。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFN-gamma，interferon gamma
缩写：IFN-γ
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：18.75 pg/mL-1200 pg/mL
灵敏度：4.7 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 86 | ||||
| Range % | 81-92 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-103 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-108 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 96-105 | |||||
回收率：
| The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 93-102 | ||||
| EDTA plasma (n=4) | 90 | 86-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected12002.0752.0512.0631.9656001.4651.4411.4531.3553001.0691.0451.0570.9591500.6550.6220.6390.541750.4000.3870.3940.29637.50.3200.2950.3080.21018.750.2050.2230.2140.11600.1010.0950.098 | ||||||
货期：3-5 working days
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