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产品描述：鱼γ干扰素(IFN-γ)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFNG含量。干扰素-γ（IFNG）是一种重要的细胞因子，主要由活化的T细胞和NK细胞产生，具有调节免疫状态、介导细胞免疫、增强吞噬功能等作用。其研究机制涉及JAK-STAT通路，通过激活下游信号级联，介导多种生物反应，如抗病毒、抗肿瘤和免疫调节。试剂盒检测范围为20 pg/mL-4000 pg/mL，适用于水产动物免疫机制研究、感染性疾病模型构建、疫苗效力评估或养殖环境中鱼类免疫状态监测等场景本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IFN-gamma，interferon gamma
缩写：IFN-
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：20 pg/mL-4000 pg/mL
灵敏度：10 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 84 | ||||
| Range % | 80-88 | |||||
| 1:2 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:4 | Average % | 100 | ||||
| Range % | 97-103 | |||||
| 1:8 | Average % | 53 | ||||
| Range % | 80-86 | |||||
回收率：
| The recovery of fish IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 91 | 87-96 | ||||
| EDTA plasma (n=4) | 96 | 90-101 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average40000.1550.1580.15710000.3110.3210.3163200.4590.4680.464800.8610.8420.852201.4051.4351.42002.4582.3112.385 | ||||||
货期：3-5 working days
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