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产品描述：鸡免疫球蛋白A(IgA)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆样本中的IgA含量。IgA即免疫球蛋白 A，是人体重要抗体，存在于黏膜表面等，在抵御病原体入侵中发挥重要作用。其研究机制主要聚焦于与疾病关系，如通过检测 IgA 水平辅助诊断某些感染、自身免疫病，也探究其作为靶点开发治疗性药物，以调节免疫。试剂盒检测范围为781.25 ng/mL-50000 ng/mL，适用于家禽免疫学研究，如评估疫苗免疫效果、监测传染性疾病（如新城疫、禽流感）感染后的抗体动态变化，亦可用于营养免疫学实验中饲料添加剂对黏膜免疫功能的调控研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IgA
种属：Gallus gallus (Chicken)
样本类型：serum, plasma
检测范围：781.25 ng/mL-50000 ng/mL
灵敏度：390 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 97 | ||||
| Range % | 93-105 | |||||
| 1:200 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:400 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:800 | Average % | 88 | ||||
| Range % | 82-96 | |||||
回收率：
| The recovery of chicken IgA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-102 | ||||
| EDTA plasma (n=4) | 102 | 98-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average500000.0720.0710.072250000.1340.1360.135125000.2310.2420.23762500.4260.4350.43131250.6660.6750.6711562.50.9380.9270.933781.251.2481.1471.19801.6951.5941.645 | ||||||
本试剂盒所含材料：
A 96-well Assay plate --The 96-well plate has been pre-coated with chicken IgA.
Standard(10 x concentrate) (1 x 200 μl) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve.
HRP-conjugated IgA antibody(100 x concentrate) (1 x 60 μl) --Bind to the IgA, and HRP catalyzes the TMB to elicit a chromogenic reaction.
HRP-conjugate Diluent (1 x 10 ml) --Dilute the HRP-conjugated IgA antibody solution.
Sample Diluent (2 x 20 ml) --Reconstitute the standard and dilute the sample to an appropriate concentration.
Wash Buffer (25x concentrate) (1 x 20 ml) --Wash away unbound or free substances.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm - 570 nm.
An incubator that can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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