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产品描述：鹿胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF-1含量。IGF-1（胰岛素样生长因子-1）是一种由IGF1基因编码的蛋白质，与胰岛素具有相似的结构和功能。它主要在肝脏合成，并通过生长激素（GH）的调节促进生长和发育。IGF-1通过激活IGF-1受体，在多个组织和器官中调节细胞增殖、分化和凋亡。其研究机制涉及IGF-1与受体的结合，以及通过多种信号通路（如PI3K/AKT和MEK/ERK）影响细胞功能。试剂盒检测范围为1 ng/mL-200 ng/mL，科研人员可将其应用于鹿科动物生长发育研究、营养干预实验、繁殖生物学分析以及代谢相关疾病模型的机制探索，例如评估不同饲养条件下IGF - 1的动态变化，或探究其在组织再生修复中的作用。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IGF-1
Uniprot No.：H9BDM1
种属：Deer
样本类型：serum, plasma, tissue homogenates
检测范围：1 ng/mL-200 ng/mL
灵敏度：0.5 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of deer IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 98 | ||||
| Range % | 92-104 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 89-104 | |||||
| 1:4 | Average % | 99 | ||||
| Range % | 87-107 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of deer IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 89-98 | ||||
| EDTA plasma (n=4) | 96 | 90-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average2000.1750.1740.175500.3330.3160.325160.6220.6400.63141.0401.0481.04411.4161.3891.40302.5772.6332.605 | ||||||
货期：3-5 working days
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