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产品描述：鱼胰岛素样生长因子1(IGF-1)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IGF-1含量。IGF-1即胰岛素样生长因子-1，它在细胞的生长、分化和增殖等生理过程中发挥重要作用。其研究机制涉及与相应受体结合，激活下游信号通路，影响细胞的存活与功能。目前在生长发育、代谢等多方面研究广泛，也与某些疾病的发生发展相关。试剂盒检测范围为25 pg/mL-2000 pg/mL，可为水产动物生理研究、养殖管理优化以及环境胁迫响应机制探索提供可靠工具，特别适用于实验室条件下鱼类生长性能评估、饲料效应分析以及激素调控网络研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：igf1Insulin-like growth factor I ELISA Kit; IGF-I ELISA Kit; Somatomedin ELISA Kit
缩写：IGF-1
Uniprot No.：Q02815
种属：Fish
样本类型：serum, plasma, tissue homogenates
检测范围：25 pg/mL-2000 pg/mL
灵敏度：31.25 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of fish IGF-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 91-99 | |||||
| 1:4 | Average % | 102 | ||||
| Range % | 98-106 | |||||
| 1:8 | Average % | 87 | ||||
| Range % | 82-93 | |||||
回收率：
| The recovery of fish IGF-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 90-99 | ||||
| EDTA plasma (n=4) | 88 | 82-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 20000.3410.3430.342 7500.4760.4840.480 3750.6820.6360.659 1251.0671.0721.070 251.4881.5671.528 01.9551.9951.975 | ||||||
货期：3-5 working days
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