首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人胰岛素样生长因子结合蛋白5(IGFBP-5)酶联免疫试剂盒，双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液样本中的IGFBP5含量。IGFBP5，即胰岛素样生长因子结合蛋白5，是一种在多种细胞类型中分泌的磷酸化和糖基化蛋白，被认为是IGFBP家族中最保守的成员。它通过调节IGF信号通路，影响细胞生长、增殖和分化。IGFBP5在生理和病理过程中发挥重要作用，如参与肿瘤生长、糖尿病肾脏病进展等。试剂盒检测范围为0.156 ng/mL-10 ng/mL，适用于体外研究IGFBP - 5在生长发育、代谢性疾病机制、癌症进展等领域的表达调控及生物学功能，尤其适合探索其在细胞模型或动物模型中的分泌动态及信号通路关联，为分子机制研究提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IBP 5 ELISA Kit; IBP-5 ELISA Kit; IBP5 ELISA Kit; IBP5_HUMAN ELISA Kit; IGF binding protein 5 ELISA Kit; IGF BP5 ELISA Kit; IGF-binding protein 5 ELISA Kit; IGFBP 5 ELISA Kit; IGFBP-5 ELISA Kit; IGFBP5 ELISA Kit; Insulin like growth factor binding protein 5 ELISA Kit; Insulin-like growth factor-binding protein 5 ELISA Kit
缩写：IGFBP5
Uniprot No.：P24593
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human IGFBP-5 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||
| Sample | Serum(n=4) | ||||
| 1:100 | Average % | 92 | |||
| Range % | 84-95 | ||||
| 1:200 | Average % | 105 | |||
| Range % | 97-108 | ||||
| 1:400 | Average % | 94 | |||
| Range % | 88-97 | ||||
| 1:800 | Average % | 98 | |||
| Range % | 94-101 | ||||
回收率：
| The recovery of human IGFBP-5 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||
| Sample Type | Average % Recovery | Range | |||
| Serum (n=5) | 93 | 86-97 | |||
| EDTA plasma (n=4) | 93 | 88-96 | |||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||
| ng/mlOD1OD2AverageCorrected102.6132.5572.5852.49351.8041.8181.8111.7192.50.9780.9660.9720.8801.250.5610.5840.5730.4810.6250.3240.3040.3140.2220.3120.2510.2340.2430.1510.1560.1430.1350.1390.04700.0910.0930.092 | |||||
货期：3-5 working days
×
