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产品描述：犬白介素10(IL-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是一种具有重要生物学意义的靶点。它是免疫调节细胞因子，具有抗炎特性。研究机制上，IL10可抑制促炎细胞因子产生，调节免疫细胞功能。对其深入研究有助于理解免疫相关疾病发病机制，为开发新治疗方法提供方向。试剂盒检测范围为1.56 pg/mL-100 pg/mL，适用于犬类免疫机制研究、炎症性疾病模型评估、疫苗或药物干预效果分析等科研场景，为探索IL - 10在感染、肿瘤或自身免疫性疾病中的调控机制提供可靠数据支持本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL10 ELISA Kit; Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：IL10
Uniprot No.：P48411
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 pg/mL-100 pg/mL
灵敏度：0.39 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 92-108 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 89 | ||||
| Range % | 84-96 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 87-98 | |||||
回收率：
| The recovery of dog IL-10spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 85-95 | ||||
| EDTA plasma (n=4) | 92 | 87-96 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected1002.9542.9672.9612.853502.3882.3982.3932.285251.7581.7861.7721.66412.51.0351.1091.0720.9646.250.5020.5460.5240.4163.120.2710.2670.2690.1611.560.1840.1960.1900.08200.1120.1030.108 | ||||||
货期：3-5 working days
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