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产品描述：豚鼠白介素10(IL-10)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL10含量。IL10是重要的细胞因子，其背景在于它具有免疫调节作用，能抑制多种促炎因子释放。研究机制方面，主要通过与其受体结合，激活下游信号通路，调控免疫细胞活性和功能，从而在炎症、自身免疫病等疾病研究中备受关注。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研场景下豚鼠感染性疾病模型、过敏反应研究、免疫调节剂开发等领域的IL-10表达水平分析，尤其适合评估实验药物或生物制剂对免疫通路的影响。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL10Interleukin-10 ELISA Kit; IL-10 ELISA Kit; Cytokine synthesis inhibitory factor ELISA Kit; CSIF ELISA Kit
缩写：IL10
Uniprot No.：Q9Z1Y5
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 85-95 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-100 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-100 | |||||
回收率：
| The recovery of guinea pig IL-10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 89-101 | ||||
| EDTA plasma (n=4) | 97 | 91-105 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.8942.9032.8992.78010002.1072.1152.1111.9925001.2331.2411.2371.1182500.7560.7670.7620.6431250.4430.4510.4470.32862.50.2890.2990.2940.17531.250.1840.1970.1910.07200.1180.1200.119 | ||||||
货期：3-5 working days
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