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产品描述：绵羊白介素12(IL-12)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12含量。IL12是重要的细胞因子，在免疫调节中发挥关键作用。它能促进T细胞和NK细胞的活化和增殖，增强机体免疫应答。研究聚焦其信号传导机制、与疾病关联。深入了解IL12机制，有望为免疫相关疾病如肿瘤、自身免疫病治疗带来新策略。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本产品适用于绵羊免疫相关疾病的机制研究，如感染性疾病模型中细胞免疫应答强度的动态监测、疫苗效力评估实验中的Th1型免疫激活分析，或炎症性疾病研究中的细胞因子网络调控解析，为兽医科学及比较医学研究提供精准的科研工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：N/A
缩写：IL-12
种属：Ovis aries (Sheep)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 87-94 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 99-107 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 87-99 | |||||
| 1:8 | Average % | 91 | ||||
| Range % | 88-96 | |||||
回收率：
| The recovery of sheep IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 96 | 92-103 | ||||
| EDTA plasma (n=4) | 90 | 86-94 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.5992.7802.6902.59610002.1772.2612.2192.1255001.6211.7301.6761.5822501.1131.0561.0850.9911250.7560.7820.7690.67562.50.3990.4110.4050.31131.250.2800.2970.2890.19500.0970.0900.094 | ||||||
货期：3-5 working days
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