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产品描述：鸡白介素12(IL-12/70)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL12/70含量。IL12/70作为靶点，IL-12和IL-70在免疫调节中发挥关键作用。针对它的研究聚焦于通过阻断其信号通路，调节免疫细胞活性及功能来治疗疾病，如自身免疫病、肿瘤等，旨在抑制异常免疫反应或增强抗肿瘤免疫。试剂盒检测范围为0.78 pg/mL-200 pg/mL，适用于家禽免疫机制研究、疫苗效力评估、病原感染模型中的细胞因子动态监测等科研场景，为禽类免疫相关疾病的分子机制探索提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-12/70
种属：Gallus gallus (Chicken)
样本类型：serum, plasma, tissue homogenates
检测范围：0.78 pg/mL-200 pg/mL
灵敏度：0.78 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-12/70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 95 | ||||
| Range % | 88-98 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 90-98 | |||||
| 1:4 | Average % | 92 | ||||
| Range % | 87-98 | |||||
| 1:8 | Average % | 96 | ||||
| Range % | 92-101 | |||||
回收率：
| The recovery of chicken IL-12/70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-99 | ||||
| EDTA plasma (n=4) | 92 | 88-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2Average 2000.1920.1880.190 500.2730.2810.277 12.50.5850.5650.575 3.120.8760.8240.850 0.781.4241.4571.441 02.2652.2332.249 | ||||||
货期：3-5 working days
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