| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SNRNP25 |
| Uniprot No | Q9BV90 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-132aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMDVFQEGLAMVVQDPLLCDLPIQVTLEEVN SQIALEYGQAMTVRVCKMDGEVMPVVVVQSATVLDLKKAI QRYVQLKQEREGGIQHISWSYVWRTYHLTSAGEKLTEDRKKLRDYGIRNR DEVSFIKKLRQK |
| 预测分子量 | 17 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3条关于SNRNP25重组蛋白的虚构参考文献(仅供示例，实际文献请通过学术数据库查询)：
1. **"Recombinant SNRNP25 expression and its role in spliceosome assembly"**
*作者：Chen L, et al.*
摘要：研究利用大肠杆菌系统重组表达SNRNP25蛋白，优化纯化条件后通过体外剪接实验证明其参与U12型内含子剪接体的组装过程，揭示了其在次要剪接通路中的功能。
2. **"Structural analysis of SNRNP25 reveals a conserved RNA-binding motif critical for pre-mRNA splicing"**
*作者：Wang Y, et al.*
摘要：通过X射线晶体学解析SNRNP25重组蛋白的三维结构，发现其N端含有保守的RNA结合结构域，突变实验证实该区域对剪接体稳定性和pre-mRNA识别具有关键作用。
3. **"SNRNP25 deficiency disrupts zebrafish neural development via aberrant mRNA splicing"**
*作者：Kim J, et al.*
摘要：利用重组SNRNP25蛋白进行斑马鱼胚胎显微注射实验，证明该蛋白缺失导致神经相关基因(如neurod1)的可变剪接异常，进而引发神经管发育缺陷。
(注：以上文献信息为模拟生成，实际研究请参考PubMed、Web of Science等平台的最新论文)
SNRNP25 is a protein component of the U12 minor spliceosome, a macromolecular complex responsible for catalyzing the removal of rare U12-type introns from precursor messenger RNAs (pre-mRNAs) in eukaryotic cells. As a member of the small nuclear ribonucleoprotein (snRNP) family, it plays a critical role in alternative splicing, a process essential for generating transcriptomic and proteomic diversity. The U12-dependent splicing machinery operates on a subset of introns (∼0.5% in humans) characterized by distinct splice site sequences and branchpoint motifs, complementing the predominant U2-dependent major spliceosome.
The SNRNP25 gene encodes a 25 kDa protein that contributes to spliceosome assembly and catalytic activation through interactions with other snRNP components and splicing factors. Structural studies suggest it participates in stabilizing snRNA-protein interactions within the U12 snRNP particle. Dysregulation of SNRNP25 has been implicated in developmental defects and diseases linked to splicing errors, particularly those affecting genes containing U12-type introns involved in cell cycle regulation and neuronal functions.
Recombinant SNRNP25 protein is typically produced in bacterial or mammalian expression systems for functional studies. Its purified form enables biochemical characterization of spliceosome assembly mechanisms, enzymatic activity assays, and identification of interacting partners. Researchers utilize this recombinant tool to investigate splicing fidelity, dissect regulatory networks in gene expression, and model pathogenic mutations associated with spinal muscular atrophy-like disorders or cancer. Recent applications also include high-throughput screening for compounds targeting spliceosomal components and developing diagnostic biomarkers for splicing-related pathologies.
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