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产品描述：犬白介素18(IL-18)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL18含量。IL18是一种细胞因子，属于白细胞介素家族。它在免疫反应中发挥重要作用，能调节Th1、NK等细胞的活性。研究机制上，IL18可通过与受体结合激活下游信号通路，参与炎症反应和免疫调节，其异常表达与多种疾病相关，是药物研发的潜在靶点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研领域对犬类炎症性疾病（如败血症、自身免疫病）、肿瘤免疫微环境或疫苗免疫效果的研究，支持多类型样本同步检测，为动物实验中的免疫指标分析提供标准化工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL18 ELISA Kit; IGIF ELISA Kit; Interleukin-18 ELISA Kit; IL-18 ELISA Kit; Interferon gamma-inducing factor ELISA Kit; IFN-gamma-inducing factor ELISA Kit; Interleukin-1 gamma ELISA Kit; IL-1 gamma ELISA Kit
缩写：IL18
Uniprot No.：Q9XSR0
种属：Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：12.5 pg/mL-800 pg/mL
灵敏度：3.12 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-18 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 104 | ||||
| Range % | 100-109 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 91-105 | |||||
| 1:4 | Average % | 90 | ||||
| Range % | 85-94 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of dog IL-18 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-99 | ||||
| EDTA plasma (n=4) | 87 | 80-91 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected8002.6752.5522.6142.4394002.0652.0762.0711.8962001.3721.4241.3981.2231000.7260.7390.7330.558500.4710.5040.4880.313250.3800.3590.3700.19512.50.2710.2590.2650.09000.1730.1770.175 | ||||||
货期：3-5 working days
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