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产品描述：豚鼠白介素1β(IL1B)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL1B含量。IL1B，即白介素-1β，是一种重要的促炎细胞因子，主要由单核细胞、巨噬细胞和树突状细胞产生。它在炎症和免疫反应中起关键作用，通过激活IL-1受体和下游信号通路，调节细胞增殖、分化和凋亡。IL1B的产生和活性受多种炎症刺激调控，如微生物产物和内源性危险相关分子。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于豚鼠模型的基础科研领域，如炎症机制研究、免疫相关疾病动物模型分析、药物或化合物抗炎效果评估等场景，为探索豚鼠在病原体感染、创伤修复或免疫干预实验中IL1β的调控机制提供可靠工具，满足实验室对炎症标志物的精准定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
缩写：IL1B
Uniprot No.：Q9WVG1
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL1B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 93 | ||||
| Range % | 90-98 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 85-100 | |||||
| 1:4 | Average % | 97 | ||||
| Range % | 92-102 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 85-98 | |||||
回收率：
| The recovery of guinea pig IL1B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 92 | 88-95 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.4002.4572.4292.2955001.9551.9631.9591.8252501.3241.3511.3381.2041250.8380.8290.8340.70062.50.5290.5560.5430.40931.20.3340.3410.3380.20415.60.2320.2380.2350.10100.1340.1330.134 | ||||||
货期：3-5 working days
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