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产品描述：猪白介素1β (IL-1β)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IL1B含量。IL1B，即白细胞介素-1β，是一种强效的促炎细胞因子，主要由单核细胞、巨噬细胞和树突状细胞产生。它在炎症和免疫反应中起关键作用，通过炎症小体激活和蛋白水解过程产生活性形式，进而调节多种细胞活动，包括细胞增殖、分化和凋亡。试剂盒检测范围为3.9 pg/mL-250 pg/mL，该产品可广泛应用于动物医学研究领域，包括但不限于感染性疾病模型的炎症反应评估、免疫调节药物开发中的疗效监测，以及疫苗研发过程中细胞因子动态变化分析，为科研人员提供稳定可靠的检测平台。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：IL1BInterleukin-1 beta ELISA kit; IL-1 beta ELISA kit
缩写：IL1B
Uniprot No.：P26889
种属：Sus scrofa (Pig)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：3.9 pg/mL-250 pg/mL
灵敏度：0.975 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-100 | |||||
| 1:2 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 90-110 | |||||
| 1:8 | Average % | 95 | ||||
| Range % | 85-105 | |||||
回收率：
| The recovery of pig IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 83-104 | ||||
| EDTA plasma (n=4) | 98 | 90-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected2502.6752.8152.7452.5971252.1542.1472.1512.00362.51.3121.3351.3241.17631.250.7880.7640.7760.62815.60.3770.3560.3670.2197.80.2640.2690.2670.1193.90.1900.1880.1890.04100.1530.1430.148 | ||||||
货期：3-5 working days
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