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产品描述：豚鼠白介素2(IL-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-2含量。IL-2是一种重要的细胞因子，在免疫系统中扮演关键角色。它能促进T细胞、B细胞的增殖分化，增强自然杀伤细胞活性等。科研人员深入研究其与免疫调节相关的信号传导通路等机制，为开发免疫治疗药物、攻克相关疾病提供了理论基础。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，适用于科研领域探究豚鼠感染模型中的免疫激活状态、评估疫苗或免疫调节剂的干预效果，以及炎症性疾病机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-2
Uniprot No.：H0VBY4
种属：Guinea Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.8 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 102 | ||||
| Range % | 98-108 | |||||
| 1:2 | Average % | 94 | ||||
| Range % | 91-98 | |||||
| 1:4 | Average % | 103 | ||||
| Range % | 97-108 | |||||
| 1:8 | Average % | 88 | ||||
| Range % | 80-96 | |||||
回收率：
| The recovery of guinea pig IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-100 | ||||
| EDTA plasma (n=4) | 104 | 96-108 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.2302.3512.2912.15210001.7131.7941.7541.6155001.2141.2351.2251.0862500.7430.7320.7380.5991250.4950.4640.4800.34162.50.3490.3380.3440.20531.250.2550.2510.2530.11400.1390.1380.139 | ||||||
货期：3-5 working days
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