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产品描述：猪白介素22(IL-22)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IL-22含量。IL-22是重要靶点。它是一种细胞因子，参与免疫调节。其背景在于在多种疾病如炎症性肠病、皮肤病等中表达异常。研究机制方面，主要通过与受体结合，激活下游信号通路，影响细胞增殖、分化及炎症反应，为相关疾病治疗提供方向。试剂盒检测范围为78 pg/mL-5000 pg/mL，适用于动物免疫调控研究、病原感染后细胞因子表达分析、肠道屏障功能评估等科研方向，为探索猪类疾病模型、疫苗效果评价及营养干预研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：IL-22
Uniprot No.：F1SH68
种属：Sus scrofa (Pig)
样本类型：serum, plasma, tissue homogenates
检测范围：78 pg/mL-5000 pg/mL
灵敏度：19.5 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of pig IL-22 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 99 | |||||
| Range % | 94-104 | ||||||
| 1:2 | Average % | 96 | |||||
| Range % | 89-100 | ||||||
| 1:4 | Average % | 95 | |||||
| Range % | 89-98 | ||||||
| 1:8 | Average % | 103 | |||||
| Range % | 95-107 | ||||||
回收率：
| The recovery of pig IL-22 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 99 | 91-103 | |||||
| EDTA plasma (n=4) | 101 | 95-107 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| pg/mlOD1OD2AverageCorrected50002.1622.0852.1241.94825001.8751.8111.8431.66712501.4691.3831.4261.2506250.7870.7640.7760.6003120.4840.4720.4780.3021560.3150.3040.3100.134780.2310.2280.2300.05400.1780.1740.176 | |||||||
货期：3-5 working days
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